Master’s thesis · Universidad Nacional de Colombia
Genome analysis and metabolic reconstruction of the Colombia Andean potato variety
A master's thesis on genome assembly and annotation of the Colombia variety of Andean potato and the reconstruction of a genome-scale metabolic model.
From data to interpretation
The institutional abstract documents a workflow from sequencing and assembly through quality evaluation, annotation, and metabolic reconstruction.
01 PacBio HiFi
Long, high-accuracy reads: the raw material for the assembly.
02 Assembly and assessment
Overlapping reads are joined into continuous sequences; the assembly is corrected, scaffolded, and assessed for quality.
03 Annotation
Genes are predicted along the assembled sequence and assigned a function.
04 Metabolic reconstruction
Annotated genes are translated into reactions that make up a genome-scale metabolic model.
Context
The ‘Criolla Colombia’ cultivar of Solanum tuberosum L. Group Phureja motivates the creation of genomic resources for studying its biology. The thesis deposited at the National University of Colombia presents a genome assembly and a genome-scale metabolic model.
From data to model
According to the institutional abstract, the work starts with PacBio HiFi reads, followed by assembly correction and assessment, scaffolding, gene prediction and annotation, and metabolic model reconstruction. The thesis documents the tools, parameters, and results of each stage; this site is not a substitute for that documentation.
Source
Read the original Spanish-language thesis at the institutional repository. Any figures or scientific conclusions should be interpreted alongside the methods and limitations in the primary source.